2GCO
Crystal structure of the human RhoC-GppNHp complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-04 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.092, 75.645, 82.903 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.400 |
R-factor | 0.19804 |
Rwork | 0.197 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z2c |
RMSD bond length | 0.009 |
RMSD bond angle | 1.360 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.903 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.085 | 0.508 |
Total number of observations | 52822 | |
Number of reflections | 75377 | 11027 |
<I/σ(I)> | 4.3 | 1.2 |
Completeness [%] | 95.0 | 95 |
Redundancy | 5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291.15 | 30% PEG 8000, 0.2M Sodium Acetate,1.2% Inositol, 0.1M Sodium Cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |