2GCN
Crystal structure of the human RhoC-GDP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.214, 56.617, 81.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 1.850 |
| R-factor | 0.17812 |
| Rwork | 0.176 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2c |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.604 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.575 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.050 | 0.148 |
| Total number of observations | 5731 | |
| Number of reflections | 17803 | 2369 |
| <I/σ(I)> | 9.9 | 4.4 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | 20% PEG 3350, 0.1M Bis Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
