2GCJ
Crystal Structure of the Pob3 middle domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.868, 157.527, 57.787 |
| Unit cell angles | 90.00, 89.77, 90.00 |
Refinement procedure
| Resolution | 78.810 - 2.550 |
| R-factor | 0.21926 |
| Rwork | 0.215 |
| R-free | 0.30287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Wild-type protein |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.504 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 31162 | |
| Completeness [%] | 91.1 | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 21% PEG 3350, 20% Glycerol, 200mM NaCl, 50mM Ammonium Sulphate, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
