2GCB
G51S/S52T double mutant of L. casei FPGS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.620, 45.320, 84.830 |
| Unit cell angles | 90.00, 107.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.199 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.373 |
| Number of reflections | 16532 | |
| <I/σ(I)> | 10.1 | 2.9 |
| Completeness [%] | 93.6 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277 | 40 mM acetate buffer, 4% PEG4000, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
