2GC1
The crystal structure of phosphoglucose isomerase from Pyrococcus furiosus in complex with sorbitol 6-phosphate and zinc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.311, 72.857, 71.640 |
Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
Resolution | 19.920 - 1.950 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x8e |
RMSD bond length | 0.010 |
RMSD bond angle | 1.653 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.673 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.062 | 0.379 |
Total number of observations | 17653 | |
Number of reflections | 28380 | 3961 |
<I/σ(I)> | 9.6 | 2 |
Completeness [%] | 92.1 | 88.2 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 0.05 M ammonium sulphate, 0.1 M sodium acetate pH 4.6, 20% PEG monoethyl ether 2000, 5 mM D-sorbitol 6-phosphate, 5mM ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |