2GBR
Crystal Structure of the 35-36 MoaD Insertion Mutant of Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00808 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.465, 54.807, 92.627 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 4.990 - 2.000 |
| R-factor | 0.21938 |
| Rwork | 0.216 |
| R-free | 0.28971 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.237 |
| Data scaling software | d*TREK (9.5LDz) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.135 | 0.421 |
| Total number of observations | 5645 | |
| Number of reflections | 14696 | 1447 |
| <I/σ(I)> | 5.9 | 2.5 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.86 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 293.15 | 24-30% PEG 4000, 50-80 mM Cadmium Chloride, 100 mM Sodium Acetate, pH 3.9, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
