2GBM
Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-24 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.817, 49.786, 123.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.511 - 1.550 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.013 |
RMSD bond angle | 1.391 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.511 | 1.600 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.054 | 0.179 |
Number of reflections | 44135 | |
<I/σ(I)> | 20.02 | 10.7 |
Completeness [%] | 98.5 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 293.15 | 25-30% PEG 4000, 50 mM Sodium Cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K, pH 5.50 |