2GBL
Crystal Structure of Full Length Circadian Clock Protein KaiC with Phosphorylation Sites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2003-06-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.25 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 132.873, 135.576, 204.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.29000 |
Structure solution method | SAD |
RMSD bond length | 0.025 |
RMSD bond angle | 2.150 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RAVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.043 | 0.022 |
Number of reflections | 87750 | |
<I/σ(I)> | 20.6 | |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | sodium formate, glycerol, pH 4, evaporation recrystallization, temperature 291K, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | sodium formate, glycerol, pH 4, evaporation recrystallization, temperature 291K, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | sodium formate, glycerol, pH 4, evaporation recrystallization, temperature 291K, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |