2GBK
Crystal Structure of the 9-10 MoaD Insertion Mutant of Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Collection date | 2004-07-24 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.046, 45.895, 54.382 |
| Unit cell angles | 79.91, 74.99, 81.33 |
Refinement procedure
| Resolution | 5.800 - 1.990 |
| R-factor | 0.24638 |
| Rwork | 0.244 |
| R-free | 0.28668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.857 |
| Data scaling software | d*TREK (9.4L) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.800 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.084 | 0.157 |
| Total number of observations | 4262 | |
| Number of reflections | 16333 | 1603 |
| <I/σ(I)> | 9.6 | 4.2 |
| Completeness [%] | 90.7 | 89.8 |
| Redundancy | 2.73 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293.15 | 27-32% PEG 6000, 4-12% Acetone, 50 mM Sodium Cacodylate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
