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2GBJ

Crystal Structure of the 9-10 8 Glycine Insertion Mutant of Ubiquitin.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 4.2.2
Synchrotron siteALS
Beamline4.2.2
Temperature [K]100
Detector technologyCCD
Collection date2004-12-17
DetectorNOIR-1
Wavelength(s)1.12711
Spacegroup nameP 21 21 21
Unit cell lengths27.124, 53.035, 87.268
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution33.691 - 1.350
R-factor0.18998
Rwork0.188
R-free0.22589
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ubq
RMSD bond length0.012
RMSD bond angle1.416
Data scaling softwared*TREK (9.5LDz)
Phasing softwareAMoRE
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]33.6911.400
High resolution limit [Å]1.3501.350
Rmerge0.0680.503
Total number of observations10991
Number of reflections278672511
<I/σ(I)>10.82.4
Completeness [%]97.890.6
Redundancy4.984.37
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.9279.1527-32% PEG 4000, 50 mM Sodium Cacodylate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 279.15K

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