2GB7
Metal-depleted Ecl18kI in complex with uncleaved, modified DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05, 0.900, 0.9198, 0.9200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.697, 96.739, 192.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.475 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.340 |
| Number of reflections | 158490 | |
| <I/σ(I)> | 31 | 3.9 |
| Completeness [%] | 97.7 | 92.6 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 294 | 0.4M (NH4)H2PO4, pH 4.1, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 294 | 0.4M (NH4)H2PO4, pH 4.1, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 294 | 0.4M (NH4)H2PO4, pH 4.1, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
