2GAX
Structure of Protein of Unknown Function Atu0240 from Agrobacteriium tumerfaciencs str. C58
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2005-10-12 |
Detector | SBC-2 |
Wavelength(s) | 0.97945 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.965, 68.998, 120.811 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.410 - 1.801 |
R-factor | 0.2475 |
Rwork | 0.247 |
R-free | 0.28416 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.227 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.412 | 1.847 |
High resolution limit [Å] | 1.801 | 1.801 |
Number of reflections | 24976 | |
Completeness [%] | 97.1 | 86.24 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Ammonium sulphate, 0.1M Tris, 25%P3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |