2GAU
Crystal structure of transcriptional regulator, Crp/Fnr family from Porphyromonas gingivalis (APC80792), Structural genomics, MCSG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 76.723, 76.723, 86.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.350 - 1.900 |
R-factor | 0.21058 |
Rwork | 0.208 |
R-free | 0.25837 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.221 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.354 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.115 | 0.897 |
Number of reflections | 20641 | |
<I/σ(I)> | 25.4 | 1.42 |
Completeness [%] | 98.6 | 91.5 |
Redundancy | 14.8 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M succinic acid, 15% PEG 3350 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |