2GAN
Crystal Structure of a Putative Acetyltransferase from Pyrococcus horikoshii, Northeast Structural Genomics Target JR32.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97904 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.706, 76.660, 45.005 |
Unit cell angles | 90.00, 100.32, 90.00 |
Refinement procedure
Resolution | 24.410 - 2.100 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.25400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.410 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.090 | 0.340 |
Number of reflections | 49028 | |
<I/σ(I)> | 14.72 | 3.85 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 4.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10 mM Tris (pH 7.5), 16% PEG3350, 350 mM ammonium sulfate, and 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 291K |