2GAJ
Structure of Full Length Topoisomerase I from Thermotoga maritima in monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2815 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.049, 142.714, 127.638 |
| Unit cell angles | 90.00, 90.84, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.950 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gai |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.999 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.120 | 0.440 |
| Number of reflections | 116615 | |
| <I/σ(I)> | 8.2 | 2.7 |
| Completeness [%] | 99.6 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 34 % Jeffamine M-600, 100 mM MES/NaOH, 64 mM Sodium Citrate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
