2GAC

T152C MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-03
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	46.200, 97.300, 61.800
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.233
Rwork	0.233
R-free	0.28000
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	24.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.180
High resolution limit [Å]	2.100	2.100
Rmerge		0.189 *
Total number of observations	59059 *
Number of reflections	30530
<I/σ(I)>	8.3	4.2
Completeness [%]	0.1 *	77.8
Redundancy	1.93	1.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3300	15 (%(v/v))
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir	sodium azide	0.1 (%)