2GA1

Crystal structure of a duf433 member protein (ava_0674) from anabaena variabilis atcc 29413 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.918381, 0.978489, 0.979094
Spacegroup name	P 21 21 21
Unit cell lengths	46.980, 65.120, 86.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.640 - 2.000
R-factor	0.178
Rwork	0.176
R-free	0.21800
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.485
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.640		2.070
High resolution limit [Å]	2.000	3.420	2.000
Rmerge	0.056	0.037	0.342
Number of reflections	18535	3430	3012
<I/σ(I)>	8.86	17.2	2.33
Completeness [%]	99.1	99.2	97.3
Redundancy	3.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	6.5	277	20.0% Glycerol, 0.16M Mg(oAc)2, 16.0% PEG-8000, 0.1M Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K