2GA0
Variable Small Protein 1 of Borrelia turicatae (VspA or Vsp1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 241.835, 69.105, 87.578 |
| Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yjg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.544 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.042 | 0.147 |
| Number of reflections | 38032 | 3523 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 98.9 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 28% (w/v) polyethylene glycol (PEG), 80 mM Tris HCl, 15% (v/v) glycerol, 100 mM NiCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
