2G9U
The crystal structure of glycogen phosphorylase in complex with (3R,4R,5R)-5-hydroxymethyl-1-(3-phenylpropyl)-piperidine-3,4-diol and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8063 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.522, 128.522, 116.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.910 - 2.150 |
| R-factor | 0.1914 |
| Rwork | 0.190 |
| R-free | 0.21844 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1hlf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.074 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.910 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 52635 | |
| <I/σ(I)> | 14 | 4.3 |
| Completeness [%] | 98.4 | 99.1 |
| Redundancy | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 287 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 287K |
