2G9R
The crystal structure of glycogen phosphorylase b in complex with (3R,4R,5R)-5-hydroxymethyl-1-(3-phenylpropyl)-piperidine-3,4-diol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2005-11-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8063 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.356, 128.356, 116.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.910 - 2.070 |
R-factor | 0.19004 |
Rwork | 0.188 |
R-free | 0.21955 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1hlf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.039 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.910 | 2.110 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 59039 | |
<I/σ(I)> | 16.9 | 4.4 |
Completeness [%] | 99.2 | 99 |
Redundancy | 5.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 287 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 287K |