2G9E
Protonation-mediated structural flexibility in the F conjugation regulatory protein, TRAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2005-11-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | I 4 |
| Unit cell lengths | 50.939, 50.939, 46.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.010 - 1.800 |
| R-factor | 0.18511 |
| Rwork | 0.182 |
| R-free | 0.24459 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7o |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.193 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.010 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.362 |
| Number of reflections | 5552 | |
| <I/σ(I)> | 25.1 | 2.38 |
| Completeness [%] | 98.6 | 84.1 |
| Redundancy | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 30% PEG 400, 0.1M MES PH 6.5, 2MM DTT, 0.5M AMMONIUM ACETATE , pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
