2G9C
Modified pyrimidines Specifically bind the purine riboswitch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9160 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.990, 35.100, 41.800 |
| Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.700 |
| R-factor | 0.231 |
| Rwork | 0.195 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 26388 | |
| Completeness [%] | 99.3 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
