2G8W

B. halodurans RNase H catalytic domain E188A mutant in complex with Ca2+ and RNA/DNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2005-03-07
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	81.671, 37.091, 61.980
Unit cell angles	90.00, 96.74, 90.00

Refinement procedure

Resolution	30.000 - 2.050
Rwork	0.215
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbi
RMSD bond length	0.004
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.150
High resolution limit [Å]	2.050	2.050
Number of reflections	10677
Completeness [%]	90.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	278	12% isopropanol, 0.1M MES 6.0, 0.3M CaAc2, VAPOR DIFFUSION, SITTING DROP, temperature 278K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	isopropanol
2	1	1	MES
3	1	1	CaAc2
4	1	1	H2O
5	1	2	MES
6	1	2	CaAc2