2G8R
The crystal structure of the RNase A- 3-N-piperidine-4-carboxyl-3-deoxy-ara-uridine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8068 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.174, 32.530, 72.512 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.19266 |
| Rwork | 0.191 |
| R-free | 0.23035 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g8q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.301 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 24227 | |
| <I/σ(I)> | 4.5 | 3.2 |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 19.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279 | 20mM SODIUM CITRATE BUFFER AND 20% PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
