2G8Q
The crystal structure of RNase A from monoclinic crystals at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8068 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.983, 32.551, 72.355 |
| Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.18671 |
| Rwork | 0.185 |
| R-free | 0.21840 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.188 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 37534 | |
| <I/σ(I)> | 3.7 | 3.7 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279 | 20 MM SODIUM CITRATE BUFFER AND 20 % PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
