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2G8G

Structurally mapping the diverse phenotype of Adeno-Associated Virus serotype 4

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE F1
Synchrotron siteCHESS
BeamlineF1
Temperature [K]100
Detector technologyCCD
DetectorADSC QUANTUM 4
Wavelength(s)0.916
Spacegroup nameI 2 2 2
Unit cell lengths339.600, 319.200, 285.000
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.000 - 3.200
Rwork0.263
R-free0.27300
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1fpv
RMSD bond length0.008
RMSD bond angle1.601
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareGLRF
Refinement softwareCNS (1.1)
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]3.200
Number of reflections163565
<I/σ(I)>4.41
Completeness [%]75.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5298virus concentrations ranging from 2 to 4 mg/ml in 20 mM Tris HCl, pH 7.5, with 2 mM MgCl, 150 mM NaCl and 1 1.5% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K

217705

PDB entries from 2024-03-27

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