2G8G
Structurally mapping the diverse phenotype of Adeno-Associated Virus serotype 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.916 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 339.600, 319.200, 285.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| Rwork | 0.263 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fpv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.601 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 163565 |
| <I/σ(I)> | 4.41 |
| Completeness [%] | 75.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | virus concentrations ranging from 2 to 4 mg/ml in 20 mM Tris HCl, pH 7.5, with 2 mM MgCl, 150 mM NaCl and 1 1.5% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
