2G88
MSRECA-dATP COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 108.212, 108.212, 72.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.400 - 3.200 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.400 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.103 | 0.530 |
| Number of reflections | 7941 | |
| <I/σ(I)> | 6.3 | 1.7 |
| Completeness [%] | 97.5 | 100 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M citrate phosphate, 30% 4000 PEG, 0.1M sodium citrate, 10% glycerol, 0.1M Nacl, 0.2M ammonium acetate, 0.025 DTT, 0.025 sodium azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
