2G7E
The 1.6 A crystal structure of Vibrio cholerae extracellular endonuclease I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.520, 64.160, 75.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.380 - 1.600 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ouo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.486 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.384 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.389 |
| Total number of observations | 17566 | |
| Number of reflections | 26728 | 3846 |
| <I/σ(I)> | 9.1 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 25% PEG 4000, 0.1 M Na acetate pH 4.6, 0.3 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
