2G79
Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2004-11-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.493, 46.325, 73.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.190 - 1.690 |
| R-factor | 0.161 |
| Rwork | 0.155 |
| R-free | 0.21400 |
| Structure solution method | Rigid Body Refinement |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.819 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.076 | 0.741 |
| Number of reflections | 17931 | 1761 |
| <I/σ(I)> | 17.03 | |
| Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% (w/v) PEG 8000 and 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% (w/v) PEG 8000 and 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
