2G75
Crystal Structure of anti-SARS m396 Antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-14 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 |
Unit cell lengths | 69.066, 69.066, 166.656 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.910 - 2.280 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dd8 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.910 | 2.370 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.081 | 0.311 |
Number of reflections | 34411 | |
<I/σ(I)> | 12 | 1.7 |
Completeness [%] | 84.8 | 42.8 |
Redundancy | 3.8 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% v/v Glycerol, 16% v/v Ethylene Glycol, 20% w/v PEG 6000, and 100 mM NaCl in 30 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |