2G6Y
Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.007, 97.160, 75.153 |
| Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
| Resolution | 29.740 - 1.600 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.940 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.740 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.480 |
| Number of reflections | 122154 | |
| <I/σ(I)> | 16.77 | 2.47 |
| Completeness [%] | 95.6 | 90.2 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.25 M sodium malonate, 100 mM sodium acetate, 2.2 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
