2G6F
Crystal Structure of the SH3 Domain of betaPIX in Complex with a High Affinity Peptide from PAK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979191 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.377, 31.427, 28.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.170 - 0.920 |
| R-factor | 0.18493 |
| Rwork | 0.184 |
| R-free | 0.19896 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.054 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 0.920 | 0.920 |
| Number of reflections | 46091 | |
| Completeness [%] | 93.1 | 93.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M HEPES, 53% MPD, 200mM [Co(NH3)]Cl3, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
