2G6E

Structure of cyclized F64L S65A Y66S GFP variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	51.090, 62.620, 71.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.300
Rwork	0.131
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.012
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.350
High resolution limit [Å]	1.300	1.300
Number of reflections	56899
<I/σ(I)>	47.8	4.6
Completeness [%]	99.7	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	50 mM MgCl2, 50 mM Hepes, 20% PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K