2G6B
Crystal structure of human RAB26 in complex with a GTP analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-02-22 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.368, 55.614, 92.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
Rwork | 0.210 |
R-free | 0.27690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1G16_A |
RMSD bond length | 0.018 |
RMSD bond angle | 1.461 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.173 | 0.068 | 0.841 |
Number of reflections | 12581 | 1383 | 1178 |
<I/σ(I)> | 4.4 | ||
Completeness [%] | 99.5 | ||
Redundancy | 7.6 | 7.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% PEG3350, 0.1M NH4SO4, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K |