2G5G
Cofacial heme binding to ChaN of Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978726 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.918, 58.790, 82.395 |
| Unit cell angles | 90.00, 119.21, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.900 |
| R-factor | 0.20902 |
| Rwork | 0.207 |
| R-free | 0.24172 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.437 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | 0.333 |
| Number of reflections | 28754 | |
| <I/σ(I)> | 8.638 | 2.699 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15% PEG 4000, 100 mM HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
