2G5D
Crystal structure of MltA from Neisseria gonorrhoeae Monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-12-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.783, 38.458, 84.591 |
| Unit cell angles | 90.00, 106.46, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.22843 |
| Rwork | 0.226 |
| R-free | 0.27324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.368 |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.074 | 0.396 |
| Number of reflections | 30354 | |
| <I/σ(I)> | 6.3 | 1.8 |
| Completeness [%] | 99.3 | 93.6 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 294 | 29% PEG 8000, 100 mM Tris pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
