2G5B
Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 166.528, 183.897, 67.081 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.234 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.580 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.106 | 0.630 |
Number of reflections | 91909 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.71 | 5.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.35 | 298 | 20% PEG 8000, 0.1 M Tris-HCl, pH 7.35, 0.4 M lithium sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 298K |