2G4F
Structure of S.olivaceoviridis xylanase Q88A/R275A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.088 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.875, 55.137, 114.183 |
| Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.650 |
| Rwork | 0.169 |
| R-free | 0.23100 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.451 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.710 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 19121 | |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 99.4 | 95.4 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 0.1M ammonium sulphate, 0.2M sodium acetate buffer, 9% PEG 8000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
