2G4D

Crystal structure of human SENP1 mutant (C603S) in complex with SUMO-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-06-29
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 43
Unit cell lengths	97.308, 97.308, 102.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.100 - 2.800
R-factor	0.248
Rwork	0.248
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.166	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.106	0.491
Total number of observations		9353
Number of reflections	23472	3444
<I/σ(I)>	4.7	1.6
Completeness [%]	99.3	99.8
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	289	0.1M CAPS, 40% PEG400, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K