2G4D
Crystal structure of human SENP1 mutant (C603S) in complex with SUMO-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-29 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 |
Unit cell lengths | 97.308, 97.308, 102.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.100 - 2.800 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.166 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.106 | 0.491 |
Total number of observations | 9353 | |
Number of reflections | 23472 | 3444 |
<I/σ(I)> | 4.7 | 1.6 |
Completeness [%] | 99.3 | 99.8 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 289 | 0.1M CAPS, 40% PEG400, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |