2G44
Human Estrogen Receptor Alpha Ligand-Binding Domain In Complex With OBCP-1M-G and A Glucocorticoid Receptor Interacting Protein 1 NR Box II Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.253, 81.907, 58.341 |
| Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.650 |
| R-factor | 0.21895 |
| Rwork | 0.217 |
| R-free | 0.25561 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zky |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.073 | 0.314 |
| Number of reflections | 14166 | |
| <I/σ(I)> | 14.03 | 3.21 |
| Completeness [%] | 97.6 | 90.1 |
| Redundancy | 4.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M AMMONIUM SULFATE 0.1M TRIS PH 7.0 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K |
