2G43
Structure of the ZNF UBP domain from deubiquitinating enzyme isopeptidase T (IsoT)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.28020, 1.2790, 1.12810 0.97623 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.380, 86.180, 59.900 |
Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.090 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.27400 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.160 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.047 | 0.064 |
Number of reflections | 17788 | |
<I/σ(I)> | 27.7 | 16.7 |
Completeness [%] | 96.7 | 97.2 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 300 | 16% polyethylene glycol 8000, 0.8 M sodium cacodylate pH 6.5, 0.16 M calcium acetate, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 300K, pH 6.50 | |
1 | 6.5 | 300 | 16% polyethylene glycol 8000, 0.8 M sodium cacodylate pH 6.5, 0.16 M calcium acetate, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 300K, pH 6.50 |