2G42
Crystal structure of a putative nucleic acid binding protein (tm0693) from thermotoga maritima at 2.28 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-15 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.174, 26.761, 73.221 |
Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
Resolution | 40.800 - 2.280 |
R-factor | 0.206 |
Rwork | 0.203 |
R-free | 0.25100 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.920 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.859 | 2.350 | |
High resolution limit [Å] | 2.280 | 3.870 | 2.280 |
Rmerge | 0.091 | 0.055 | 0.475 |
Number of reflections | 7150 | 766 | 385 |
<I/σ(I)> | 12.72 | 25.8 | 2.73 |
Completeness [%] | 94.9 | 98.7 | 66.4 |
Redundancy | 6.777 | 3.398 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 5.1 | 277 | 0.2M CaCl2, 20.0% PEG-3350, No Buffer, pH 5.1, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |