2G3V
Crystal structure of CagS (HP0534, Cag13) from Helicobacter pylori
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792, 0.9794, 0.9756, 1.0 |
| Spacegroup name | P 43 |
| Unit cell lengths | 124.360, 124.360, 55.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 38297 | |
| <I/σ(I)> | 5 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.2M KBr, 25% PEG 2000 MME, 0.1M Tris pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 283K |
