2G3O
The 2.1A crystal structure of copGFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-04 |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | P 32 |
Unit cell lengths | 145.954, 145.954, 53.767 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.710 - 2.100 |
R-factor | 0.2325 |
Rwork | 0.230 |
R-free | 0.27160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.723 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.714 | 2.155 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 74388 | |
Completeness [%] | 99.5 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 22% PEG 3350, 0.1M Sodium Acetate, 0.15M Magnesium Sulphate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |