2G3J

Structure of S.olivaceoviridis xylanase Q88A/R275A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-17
Detector	MARRESEARCH
Wavelength(s)	0.999
Spacegroup name	P 63
Unit cell lengths	119.953, 119.953, 55.216
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.163
R-free	0.22300
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.014
RMSD bond angle	1.491
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.700	2.700
Number of reflections	12287
<I/σ(I)>	12.5	4.8
Completeness [%]	97.3	98
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	2.1M ammonium dehydrogen phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	2.1M ammonium dehydrogen phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K