2G3B
Crystal structure of putative TetR-family transcriptional regulator from Rhodococcus sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-02 |
| Detector | SBC |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.890, 75.320, 100.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.460 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.626 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.081 | 0.372 |
| Number of reflections | 28941 | |
| <I/σ(I)> | 47.8 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.5M Ammonium Sulphate, 0.1M Tris, pH 8.5, 12% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
