2G33
Human Hepatitis B Virus T=4 capsid, strain adyw
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 558.400, 327.145, 562.237 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.960 |
| Rwork | 0.360 |
| R-free | 0.37200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.990 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.020 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.061 | 0.482 |
| Number of reflections | 666347 | 26503 |
| <I/σ(I)> | 10.5 | 1.3 |
| Completeness [%] | 81.1 | 64.6 |
| Redundancy | 2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 300 | PEG 5000 MME, butanediol, KCl, NaCl, bicarbonate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
