2G2R
Green-fluorescent antibody 11G10 in complex with its hapten (nitro-stilbene derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.788, 53.761, 110.390 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.750 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.25024 |
| Structure solution method | MIR |
| Starting model (for MR) | 1mrf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 25496 | |
| <I/σ(I)> | 9.1 | 1.9 |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG 4000, ammoniumsulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
