2G2P
Crystal Structure of E.coli transthyretin-related protein with bound Zn and Br
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-06 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.720, 92.030, 57.490 |
Unit cell angles | 90.00, 103.50, 90.00 |
Refinement procedure
Resolution | 17.380 - 2.100 |
R-factor | 0.17752 |
Rwork | 0.175 |
R-free | 0.22903 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f41 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.334 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.1.27) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.300 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.047 | 0.200 |
Number of reflections | 25512 | |
<I/σ(I)> | 27.1 | 10 |
Completeness [%] | 96.3 | 93.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG 550 MME, 100mM MES, 10mM ZnSO4 (Crystal soaked in crystallization buffer enriched with 100mM NaBr for 12h prior to data collection), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |