2G2C
Putative molybdenum cofactor biosynthesis protein from Corynebacterium diphtheriae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-09 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97894 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 67.822, 67.822, 114.276 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.520 - 1.500 |
| R-factor | 0.1739 |
| Rwork | 0.171 |
| R-free | 0.20210 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.535 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.510 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.073 | 0.664 |
| Number of reflections | 23460 | |
| <I/σ(I)> | 49.3 | 2.23 |
| Completeness [%] | 91.4 | 49.5 |
| Redundancy | 29 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Hepes buffer, 3 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
